Table I.
Strains and Plasmids Used in This Work
| Strain | Relevant genotype or description | Reference or source |
|---|---|---|
| C. crescentus | ||
| CB15N | Synchronizable variant strain of CB15; also known as NA1000 | Evinger and Agabian,1977 |
| CJW932 | CB15N creS::pJM21creS-flag | Ausmees et al.,2003 |
| CJW1231 | CB15N/pMR20creS-tc | This study |
| CJW1233 | CB15N ΔcreS/pMR20creS-tc | Cabeen et al.,2009 |
| CJW1474 | CB15N/pMR20creSΔT | This study |
| CJW1475 | CB15N ΔcreS/pMR20creSΔT | This study |
| CJW1478 | CB15N/pMR20creSΔR-tc | This study |
| CJW1518 | CB15N ΔcreS/pMR20creSSAT-tc | This study |
| CJW1520 | CB15N/pMR20creSΔL1-tc | This study |
| CJW1521 | CB15N ΔcreS/pMR20creSΔL1-tc | Cabeen et al.,2009 |
| CJW1522 | CB15N/pMR20creSSAT-tc | This study |
| CJW1535 | CB15N/pMR20creSΔN28-79-tc | This study |
| CJW1536 | CB15N ΔcreS/pMR20creSΔN28-79-tc | This study |
| CJW2862 | CB15N ΔcreS/pMR20creSΔR-tc | This study |
| CJW2927 | CB15N ΔcreS/pMR20creSΔN27-tc | Cabeen et al.,2009 |
| LS3812 | CB15N ΔcreS | Gitai et al.,2004 |
| E. coli | ||
| DH5α | Cloning strain | Invitrogen |
| S17-1 | RP4-2 Tc::MuKM-Tn7, for plasmid mobilization | Simon et al.,1983 |
| BL21 (DE3) | F-ompT hsdSB (rB- mB-) dcm gal (DE3) | Novagen |
| CJW1659 | BL21 (DE3)/pET29acreS | This study |
| CJW1353 | BL21 (DE3)/pET29acreSΔN27 | This study |
| CJW2054 | BL21 (DE3)/pET29acreSΔL1 | This study |
| CJW2045 | BL21 (DE3)/pET29acreSSAT | This study |
| CJW3663 | BL21 (DE3)/pET29acreSΔT | This study |
| Plasmid | Description | Reference or Source |
| pBluescriptKS+ | AmpR cloning vector | Stratagene |
| pET29a | Protein expression plasmid | Novagen |
| pET29acreS | pET29a carrying creS coding sequence | This study |
| pET29acreSΔN27 | pET29a carrying creSΔN27 coding sequence | This study |
| pET29acreSΔL1 | pET29a carrying creSΔL1 coding sequence | This study |
| pET29acreSSAT | pET29a carrying creSSAT coding sequence | This study |
| pET29acreSΔT | pET29a carrying creSΔT coding sequence | This study |
| pHL23 | KanR cloning vector | Lam et al.,2006 |
| pMR20 | TetR low-copy-number broad host range vector | Roberts et al.,1996 |
| pMR20creS-tc | pMR20 carrying creS-tc gene, coding for crescentin with a C-terminal tetracysteine tag | Cabeen et al.,2009 |
| pMR20creSΔN28-79-tc | pMR20 carrying creS-tc gene deleted for N28-79 coding sequence | This study |
| pMR20creSΔL1-tc | pMR20 carrying creS-tc ORF deleted for linker 1 coding sequence | Cabeen et al.,2009 |
| pMR20creSΔR-tc | pMR20 carrying creS-tc gene deleted for rod coding sequence | This study |
| pMR20creSΔT | pMR20 carrying creS gene deleted for tail coding sequence | This study |
| pMR20creSSAT-tc | pMR20 carrying creS-tc gene with coding sequence for Ser-Ala-Thr inserted at stutter site | This study |