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Hsp90α is present in the exosomes of four cancer cell lines. Western blot analysis of exosomes isolated from invasive cancer cell lines treated with vehicle (-) or DMA (+), which blocks the exosome pathway. Proteins were resolved by electrophoresis on 10% SDS-PAGE and transferred to a nitrocellulose membrane. Western blot was performed using hsp90α, annexin II, flotillin, and vATPase antibodies. Representative images taken from three independent experiments are shown.
