Figure 4.

Exosomes increase hsp90α dependent glioma and breast cancer cell movement. (a) SUM159 breast cancer cells were wounded and images captured immediately and 16 hours after wounding. Cells were treated with either PBS, recombinant hsp90α protein, exosomes isolated from SUM159 cells or exosomes isolated from SUM159 cells plus anti-hsp90α antibody. Representative images from three independent experiments are shown for each time point and condition. Scale bar = 150 μM (b) A172 glioma cells were wounded and images captured immediately or 16 hours after wounding. Cells were treated with either PBS, recombinant hsp90α protein, exosomes isolated from A172 cells or exosomes isolated from A172 cells plus anti-hsp90α antibody. Representative images from three independent experiments are shown for each time point and condition. Scale bar = 150 μM (c) Graphical representation of the wound healing assay with SUM159 and A172 cancer cell lines. The addition of both recombinant hsp90α protein and anti-hsp90α antibody results in a significant decrease in wound closure at 16 hours (p < 0.05, p < 0.01 respectively, two tailed t test).