Figure 5.
Extracellular hsp90α co-immunoprecipitates with tissue plasminogen activator. (a) MS/MS spectrum of the peptide (248VYTAQNPSAQALGLGK263) that was used to identify tPA as a HSP90α interacting protein. (b) Immunoprecipitation of hsp90α from the conditioned media of HT-1080 fibrosarcoma cells. Hsp90α was immunoprecipitated from the media of HT-1080 cells and resolved on 10% SDS-PAGE. Western blot analysis was performed using anti-hsp90α or anti-tPA antibodies. The input lane contains approximately 1/100th of the total media collected. "IP Ab" indicates the antibody used during immunoprecipitation (hsp90α, tPA or no antibody as the negative control). "IB Ab" indicates which antibody was used the probe the immunoblot. Representative images from three independent experiments are shown.