Table 2.
Primer pairs for amplification of marker gene sequences for RT-PCR expression and immunobead RT-PCR analyses.
| Primer Name | Primer Sequence 5'-3' |
Annealing Temperature | Product Size |
|---|---|---|---|
| ELF3 F | CTCGGAGCTCCCACTCCTCAGA | 68°C | 188 bp |
| ELF3 R | GCTCTTCTTGCCCTCGAGACAGT | ||
| EphB4 F | CCCCAGGGAAGAAGGAGAGCTG | 68°C | 251 bp |
| EphB4 R | GCCCACGAGCTGGATGACTGTG | ||
| EGFR F | TGTGAGGTGGTCCTTGGGAATTTGG | 66°C | 339 bp |
| EGFR R | TGCTGACTATGTCCCGCCACTGGA | ||
| CEA F | GGTTGGGGTTGCTCTGATATAGCAGC | 68°C | 97 bp |
| CEA R | GCTGTTGCAAATGCTTTAAGGAAGAAGC | ||
| CK19 F | GACTACAGCCACTACTACACGACC | 68°C | 743 bp |
| CK19 R | AGCCGCGACTTGATGTCCATGAGCC | ||
| EpCam F | GGACCTGACAGTAAATGGGGAAC | 68°C | 186 bp |
| EpCam R | CTCTTCTTTCTGGAAATAACCAGCAC | ||
The sequences of forward (F) and reverse (R) primers for each of the markers are shown in 5' to 3' direction and were used at the indicated annealing temperature to generate a PCR product of the size indicated in base pairs (bp).