Table 3.
Comparison of the attachment of immunobeads coated with antibodies BerEP4 and EMA to cells from four HNSCC cell lines.
| BerEP4 | EMA | |||
|---|---|---|---|---|
| Cell line | Cells with beads attached (%) | Average number of beads attached/cell | Cells with beads attached (%) | Average number of beads attached/cell |
| FaDu | 100 ± 0 | 6.4 ± 3.1 | 51.0 ± 5.0 | 2.1 ± 1.3 |
| Detroit | 98 ± 2 | 7.0 ± 3.9 | 13.7 ± 4.2 | 1.4 ± 0.7 |
| SCC-9 | 100 ± 0 | 10.0 ± 2.0 | 61.5 ± 2.5 | 2.4 ± 1.1 |
| SCC-25 | 100 ± 0 | 13.0 ± 4.0 | 30.0 ± 2.0 | 1.4 ± 0.5 |
Cells were incubated with either BerEP4- or EMA-conjugated beads and a sample viewed using a light microscope. The BerEP4 antibody recognises the EpCam protein and the EMA antibody recognises Muc1/EMA/Mucin 1. The total number of cells and the number of these cells with beads attached were counted and the results are presented as a percentage of cells with beads attached. The average number of beads attached per cell was also determined.