Fig. 2.

IL-1p and GM-CSF secretion in THP-1 cells stimulated with 2–200 nM active HRgpA (HA), inactive HRgpA (HI), active Kgp (KA), inactive Kgp (KI), active RgpB (RA), inactive RgpB (RI), 100 (xM PAR-1 AP (P1AP) or 100 (xM PAR-2 AP (P2AP), compared to a control (CTL). The conditioned medium was recovered at 18 h (dark bars) or 36 h (light bars) and analyzed for levels of IL-1p and GM-CSF and compared to controls. Data are presented as means ± SEM (n = 3). * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Comparisons between 2 groups were made by unpaired Student's t tests.