Table 1. Primers used for PCR amplification and sequencing of TSPAN12.
| Exon | Forward primer(5′-3′) | Reverve primer(5′-3′) | Amplicon size (bp) | Annealing tempeture (°C) |
|---|---|---|---|---|
| 2 |
GCTGTGGGAAGCGTGAT |
TTAGCCATGCCCTTTGG |
281 |
58 |
| 3 |
TTCAAGATGCAGCAAATGGTAAT |
GCTATGGGCAGGAAAAACTT |
329 |
58 |
| 4 |
ATGTCTTGGGTGCATTT |
AAGCGTCCCTTCTTACA |
321 |
56 |
| 5 |
AGGGCTCACTGAGATGA |
TCTTGGGCAGTTCTTTC |
382 |
55 |
| 6 |
TTAGAAGACATTCCGAGTA |
AATTGCACCAGAGGTTA |
360 |
55 |
| 7 |
TCCTTTACTACATTTCTATC |
TAGCTTTCTTCTGCTTC |
462 |
52 |
| 8 | ACAGATTGTTTGCTTTC | AGGTGTTATTTTATGGC | 505 | 52 |