Immunohistochemistry for GDF-15 in lung tissue stained with anti-GDF-15 antibody. A, Normal lung tissue. B, SSc-no PAH. C, Idiopathic pulmonary arterial hypertension. D, SSc-PAH. E, Prostate cancer tissue (positive control). (A-E, original magnification × 20). See Figure 1 legend for expansion of the abbreviations.