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. 2011 Feb 8;117(16):4304–4314. doi: 10.1182/blood-2010-04-278218

Figure 1.

Figure 1

CD70-CAR generation, cell-surface expression, and transduction of human T cells. (A) CD70-CAR was generated by fusing full-length CD27 to the signaling domain of the CD3-ζ chain, an IRES sequence, and tCD19 was included for the detection of genetically modified T cells. (B) 293T cells transfected with CD70-CAR constructs express both CD27 and the marker gene tCD19. (C) CD70-CAR expression on transduced human T cells was 45% ± 6%, as determined by staining tCD19. (D) Both CD4 and CD8 T cells were genetically modified.