Figure S4.

Akt Signaling Is Sufficient for Neuroblast Growth and Cell-Cycle Re-Entry, Related to Figure 4

24h VNC from a larva reared on a sucrose-only diet in which grh-GAL4 is driving UAS myr-Akt and UAS mCD8-GFP expression. myr-Akt expression is sufficient for neuroblast reactivation in the absence of the amino-acid stimulus.

Confocal channels have been split to allow better visualization of representative mitotic figures. All grh-Gal4-positive neuroblasts have enlarged, and many have begun to divide as evidenced by smaller, GFP-retaining, daughter cells adjacent to the neuroblasts, and pH3 staining. Mitotic pH3 nuclei can be seen in both neuroblasts (white arrows), and neuroblast progeny (yellow arrow). (Scale bars represent 20μm).