Table 2.

List of number of individuals per lake and species and summary statistics of mtDNA and microsatellites (averaged across 15 loci).

	Nicaragua		Managua
	A. citrinellus	A. labiatus	A. citrinellus	A. labiatus	Tisma Pond	Asososca León	Lake Apoyeque	Lake Xiloá	Asososca Managua	Lake Masaya	Lake Apoyo
No. Individuals	345	268	265	32	56	40	90	522	44	149	248
mtDNA sequences
Haplotype richness	17.81	12.66	15.09	14	14.86	6.75	1.33	3.22	4.77	3.42	5.29
% private haplotypes	44%	76%	40%	43%	25%	100%	2%	66%	75%	39%	100%
Distance (no. mutations)	16	13	13	10	10	5	3	10	4	9	8
Haplotype diversity	0.963 ± 0.006	0.954 ± 0.008	0.942 ± 0.008	0.867 ± 0.005	0.883 ± 0.036	0.822 ± 0.040	0.128 ± 0.0047	0.824 ± 0.021	0.588 ± 0.074	0.807 ± 0.021	0.736 ± 0.028
Nucleotide diversity	0.005 ± 0.003	0.005 ± 0.003	0.005 ± 0.003	0.003 ± 0.002	0.005 ± 0.003	0.003 ± 0.002	0.0001 ± 0.0001	0.002 ± 0.001	0.001 ± 0.001	0.003 ± 0.002	0.002 ± 0.001
Tajima's D	-2.524**	-2.102**	-2.201**	-1.845*	-1.161ns	-0.504ns	-1.364*	-1.899*	0.041ns	-1.488*	-2.181**
microsatellites
Allelic richness	12.81	11.51	10.89	10.06	10.83	4.10	6.02	9.13	5.67	9.36	7.45
Gene diversity	0.780	0.757	0.744	0.717	0.746	0.421	0.580	0.668	0.524	0.737	0.590
Fis	0.035	0.009	0.048	0.048	0.043	0.152	0.038	0.117	0.044	0.033	0.142

Haplotype and allelic richness are calculated haplotypes measures the maximum distance significant. based on the minimum population sample size (32 individuals). Distance between in mutations within each sample. Probability values: *P < 0.05, **P < 0.001, ns, non significant.