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. 2011 Apr 26;2(5):1340–1350. doi: 10.1364/BOE.2.001340

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 5 — Single frames showing 3-D fluorescence lifetime reconstructions on a (a) continuous (Media 5^{(328.7KB, AVI)}) and (b) discrete color scale (Media 6^{(328.9KB, AVI)}) of a live lysC:GFP transgenic zebrafish embryo 3 days post-fertilization. (c) A fluorescence lifetime histogram showing two clear populations corresponding to GFP and autofluorescence. Scale bar 500 μm.