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. 2011 Apr 29;2(5):1377–1393. doi: 10.1364/BOE.2.001377

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 5 — Performance versus active emitter density and intensity distribution. Shown are the results of MFA analysis of images with spatially random distributed emitters with normally distributed intensities of 300 ± 30 (a), (b), 800 ± 100 (c), (d), and 5000 ± 30 (e), (f). Localization error is calculated as the distance from the estimated position to the found position and in all cases assumes N_max = 5. The median localization error is where the cumulative distribution reaches 0.5. Localization fraction is the fraction of emitters that are correctly localized as determined by being found within either 20 nm or 50 nm from the known position.