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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Neurosci. 2010 Oct 31;13(12):1489–1495. doi: 10.1038/nn.2667

Figure 6. Expression of glr-1 cDNA bypasses the requirement for grld-1.

Figure 6

(a–c) Schematic cartoon of the rescuing constructs. Lines, introns; boxes, exons; pentagons, 3’ UTR. Note this glr-1 depiction does not contain all of the introns and exons. glr-1 genomic::glr-1 3’ UTR: all endogenous exons, introns, and 3’ UTR (a). glr-1 cDNA::unc-10 3’ UTR: all endogenous exons and introns, unc-10 3’ UTR (b). glr-1 cDNA::glr-1 3’ UTR: all endogenous exons, no introns, glr-1 3’ UTR (c). (d–g) Representative L2-stage wild-type animals (d, f) and grld-1(wy225) mutants (e, g). Asterisk, AVE cell body. Scale bar, 2 µm. (h) Effectiveness of the glr-1 constructs in rescuing the GLR-1 fluorescent phenotypes. The glrd-1(wy225) intensities were normalized to their respective expression constructs. n ≥ 19. Error bars, s.e.m. ***P < 0.001, *P < 0.05, t-test. (i) Effectiveness of the glr-1 constructs in rescuing the nose-touch behavior defect. The compared genotypes were assayed on the same days. n ≥ 20. Error bars, s.e.m.***P < 0.001, **P < 0.01, t-test.