Table 2. Details of the samples used with the Pi minimal screen (Table 1 ▶) and number of crystallization hits.
TEN, buffer consisting of 20mM 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris), 1mM ethylenediaminetetraacetic acid (EDTA), 1mM sodium azide and 200mM sodium chloride.
| Protein | Concentration (mgml1) | MW (kDa) | Source and code | Buffer/preparation | Hits |
|---|---|---|---|---|---|
| Lysozyme | 10.0 | 14.4 | Sigma L6876 | Deionized water | 56 |
| Concanavalin A | 7.0 | 26.5 | Sigma L7647 | TEN pH 8.5 | 16 |
| Glucose isomerase | 33.0 | 43.0 | Hampton HR7-102 | See product user guide | 11 |
| Xylanase | 36.0 | 21.0 | Hampton HR7-106 | See product user guide | 8 |
| Ferritin | 50150 | 440.0 | Fluka 96701 | As supplied by the manufacturer | 8 |
| Catalase | 12.6 | 62.5 | Sigma C3155 | Deionized water | 6 |
| Citrate synthase | 10.0 | 49.0 | Sigma C3260 | TEN pH 8.5 | 5 |
| Lipase B | 25.0 | 35.0 | Hampton HR7-099 | Deionized water | 4 |
| Ribonuclease A | 30.0 | 13.7 | Sigma R5503 | Deionized water | 1 |
| Thaumatin | 30.0 | 22.0 | Sigma T7638 | Deionized water | 1 |
| Sum | 116 |