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. Author manuscript; available in PMC: 2011 May 4.
Published in final edited form as: Cell Transplant. 2010 Aug 17;19(12):1645–1657. doi: 10.3727/096368910X516628

Figure 5.

Figure 5

Transplantation of insulin-expressing mouse Sertoli cells and immunohistochemical analysis of Sertoli cells transduced with the furin-modified adenoviral vector. (A) Average blood glucose values ± SEM of SCID mice after transplantation with 20 million mSC. mSC were transduced with AdCMVhInsM (filled diamonds; n = 3) or AdRSVGFP (filled squares; n = 1) at a MOI of 100. *Mean blood glucose values were significantly decreased compared to pretransplant values (p < 0.05). (B) mSC grafts were removed from SCID mice at 8 (a and b) and 30 (c and d) days after transplantation with mSC and immunostained for the SC marker GATA-4 (a and c) or insulin (b and d). The graft and kidney are separated by a dotted line. mSC cultured overnight as a monolayer on chamber slides were transduced with AdCMVhInsM at a MOI of 0 (e) or 100 (f–h) and collected after 5 (e and f), 12 (g), or 20 (h) days. Slides were fixed with 1% paraformaldehyde and immunostained for insulin (e-h). All sections were counterstained with hematoxylin. Arrow shows SC.