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. 2011 Apr 27;67(Pt 5):568–571. doi: 10.1107/S174430911100741X

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Purification of the refolded SLA-I complex (SLA-1*1502, sβ₂m and the epitope PRRSV-NSP9_TY9) by FPLC Superdex 200 16/60 Hi-Load gel-filtration and Resource Q anion-exchange chromatography (GE Healthcare). (a) Gel-filtration profile of the refolded products. Peak 1 represents the correctly refolded complex (∼44 kDa) and peak 2 represents sβ₂m. Inset: reduced SDS–PAGE gel (15%) for peak 1 and peak 2. The right column contains molecular-weight markers (kDa). (b) Results of further purification of the refolded products by anion-exchange chromatography. Peak 1 represents the SLA-I complex, which was eluted at an NaCl concentration of 16.6–20.2%. Inset: reduced SDS–PAGE gel (15%) for peak 1.