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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Clin Cancer Res. 2011 Mar 9;17(9):2767–2776. doi: 10.1158/1078-0432.CCR-10-3266

Figure 4.

Figure 4

(a) iDC (top row) or mDC (bottom row) were loaded ± 10 pfu/cell reovirus, and cultured with Mel-888 cells in the presence of blocking serum; after 48 h the cells were harvested, labelled with CD11c and analyzed for the maturation markers indicated. Plots shown are representative of 3 independent experiments. (b) PFA-fixed or live DC were loaded as in (a) and cultured with Cell Tracker Green-labelled Mel-888 cells, then harvested and labelled with CD11c. Double positive cells were identified by FACS. Plots shown are representative of 4 independent experiments. (c) reovirus-loaded cells (iDC(reo), mDC(reo)) or non-loaded cells (iDC, mDC), were cultured for 24 h with Mel-888 cells and used to prime isolated autologous T cells. Cells were harvested and a CD107 degranulation assay against Mel-888 or SKOV-3 (irrelevant) targets was performed. Plots shown are representative of 3 independent experiments; numbers indicate % of CD8 cells degranulating.