Table 2.
Contigs representing the enzymes and proteins involved in oil biosynthesis
| S.No | Annotation | No. of genes | Contig Number | Size (bp) | Functions |
|---|---|---|---|---|---|
| 1 | Pyruvate kinase | 2 | 2179, 2717 | 2013*, 1637 | Conversion of phosphoenol pyruvate to pyruvate |
| 2 | Pyruvate dehydrogenase E1a subunit | 2 | 2503, 3695 | 1707*, 1317* | Conversion of pyruvate to acetyl-CoA |
| 3 | Pyruvate dehydrogenase E1b subunit | 2 | 1782, 4154 | 1965*, 1203 | Conversion of pyruvate to acetyl-CoA |
| 4 | Pyruvate dehydrogenase E2 subunit | 3 | 1049, 1574, 11093 | 2430*, 2115, 517 | Conversion of pyruvate to acetyl-CoA |
| 5 | Pyruvate dehydrogenase E3 subunit | 2 | 1908, 3744 | 1934*, 1299 | Conversion of pyruvate to acetyl-CoA |
| 6 | Lipoate synthase | 1 | 10908 | 529 | Conversion of pyruvate to acetyl-CoA |
| 7 | Lipoyl transferase | 1 | 6252 | 864 | Conversion of pyruvate to acetyl-CoA |
| 8 | Homomeric acetyl-CoA carboxylase | 1 | 124 | 3711 | Conversion of acetyl-CoA to malonyl-CoA |
| 9 | Heteromeric acetyl-CoA carboxylase biotin carboxylase subunit | 1 | 1836 | 1972* | Conversion of acetyl-CoA to malonyl-CoA |
| 10 | Heteromeric acetyl-CoA carboxylase carboxyl transferase alpha subunit | 1 | 2789 | 1609* | Conversion of acetyl-CoA to malonyl-CoA |
| 11 | Heteromeric acetyl-CoA carboxylase carboxyl transferase beta subunit | 1 | 2079 | 1868* | Conversion of acetyl-CoA to malonyl-CoA |
| 12 | Heteromeric acetyl-CoA carboxylase biotin carboxyl carrier protein | 2 | 3458, 4048 | 1388*,1232* | Conversion of acetyl-CoA to malonyl-CoA |
| 13 | Acyl carrier protein | 2 | 13541,7450 | 437*,738* | Conversion of malonyl-CoA to malonyl-ACP |
| 14 | Beta-ketoacyl-ACP synthase III | 1 | 4145 | 1204 | Conversion of malony-ACP to 3-keto acyl ACP |
| 15 | 3-Ketoacyl-ACP reductase | 1 | 6732 | 1455* | Conversion of 3-keto acyl ACP to 3-hydroxy acyl ACP |
| 16 | Beta-ketoacyl-ACP synthase I | 1 | 1954 | 1915* | Serial conversion of butyryl-ACP to palmitoyl-ACP |
| 17 | Beta-ketoacyl-ACP synthase II | 1 | 11467 | 510 | Conversion of palmitoyl ACP to Stearoyl-ACP |
| 18 | Stearoyl-ACP desaturase | 4 | 6477,7609,2187, 11778 | 840, 727,1823*,496 | Conversion of Stearoyl-ACP to oleoyl-ACP |
| 19 | Delta-12 fatty acid desaturase | 2 | 1974, 3130 | 1914*, 1508* | Conversion of oleoyl-ACP to linoleoyl-ACP |
| 20 | Omega-3 fatty acid desaturase | 2 | 1744,1847 | 2015*,1767* | Conversion of linoleoyl-ACP to α-linolenyl-ACP |
| 21 | Fatty acyl-ACP thioesterase B | 1 | 4664 | 1098 | Conversion of saturated fatty acyl-ACP to saturated free fatty acid |
| 22 | Palmitoyl-ACP thioesterase | 1 | 7152 | 769 | Hydrolysis of Palmitoyl-ACP to palmitic acid |
| 23 | Fatty acyl-ACP thioesterase A | 1 | 5723 | 1452* | Conversion of unsaturated fatty acyl-ACP to unsaturated free fatty acid |
| 24 | Long chain acyl CoA synthetase | 4 | 910,932,1027, 9748 | 2539*,2516*, 2442*,579 | Activation free fatty acid to fatty acyl-CoA |
| 25 | Acyl-CoA binding protein | 5 | 3898,955,6476, 13592, 10829 | 1267,2497*, 840, 436, 524* | Transport of acyl-CoA to the ER |
| 26 | Glycerol-3-phosphate acyl transferase | 3 | 4004, 7597, 8581 | 1244,729,643 | Acylation of glycerol-3-phosphate to lysophosphatidic acid |
| 27 | Lysophosphatidic acid acyl transferase | 5 | 10599,6878, 11276,12982,6748 | 543, 799, 511, 462, 811 | Acylation of lysophosphatidic acid to phosphatidic acid |
| 28 | Phosphatidic acid phosphatase | 1 | 10375 | 549 | Conversion of phosphatidic acid to diacyl glycerol |
| 29 | Diacyl glycerol acyl transferase | 2 | 11532,14284 | 509,361 | Acylation of diacyl glycerol to triacyl glycerol |
| Total number of genes | 56 |
* Contigs representing full length genes