Table 3.
Name of the genes, primers used and expected size of the RT-PCR products for semi-quantitative RT-PCR
| S.No | Gene Name | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|---|
| 1 | Biotin carboxyl carrier protein I of ACCase (BCCP I) | GTCGGCTAATCTTAAAGCTATTC | TGTTTATAGCTTCACTAGTGTAC | 258 |
| 2 | Biotin carboxyl carrier protein II of ACCase (BCCP II) | GATGCTCTCATTGCAATTCTC | TAATGATAAATAACAATGAAGAAGG | 278 |
| 3 | 3-keto acyl ACP Reductase (KAR) | GTTATCTCTCCCGAAAGTGTA | CACAGTATCTGTCACCTTTTC | 306 |
| 4 | Beta-keto acyl ACP synthase I (KASI) | GCATCTGGCTTGTCTCCAT | GCAGAAGAACACAATTTTGATAC | 402 |
| 5 | Acyl ACP thioesterase (FATA) | AATAATGTAGATTTCTTTATTTGTGT | AGTAAACGTAAAACAATACAGTTGAT | 285 |
| 6 | ω-3-fatty acid Desaturase (ω-3-FAD) | AAAGCTGCAAAATTTTTTATCTGCA | CCCTCTCAAATCCAATCCAA | 215 |
| 7 | Diacyl glycerol kinase (DGK) | CGGCTATTCGGTTGGAAATAA | GATTTTTGATACAACAAATTACCAGT | 296 |
| 8 | Delta12-fatty acid desaturase(Δ-12 FAD) | CTGATCAAAGCAGAGGTGTGT | CTCTGAATTTGAATTTTTATAAACC | 301 |
| 9 | Acyl-CoA thioesterase (ACT2) | CACGTGGTTTTGTATCTGGC | ACCCCGGCTGCTAGAATCAA | 277 |
| 10 | Stearoyl-ACP desaturase (SAD) | ATCCAATGTGCCATTCAGCTG | ACCTCCTGCTCGAGAATAGA | 419 |
| 11 | Pyruvate kinase(PK) | GTCTCATGACAGGGTAGTTGT | ACCTGCCTGCCATGGCATTC | 264 |
| 12 | Actin | CAAGTCATCACCATTGGAGCA | CCTTGGAAATCCACATCTGTT | 342 |