Figure 3. Zinc chloride reversed the inhibition of MMP-2 activity by ellagic acid in human endothelial cells.

A. EA dose-dependently inhibited the MMP-2 activity of HUVECs. B. Co-incubation of 5 µM EA with ZnCl₂ ranged from 0–100 µM for 24 hours reversed the inhibition of MMP-2 activity of HUVECs by EA. C. HUVECs were treated first with 5 µM EA alone and the culture media were replaced at 1, 3, 6 and 12 hours later by fresh culture media containing 100 µM ZnCl₂. Conditioned media were collected 24 hours later and used for the gelatin zymography analysis.