Figure 7. Zinc reversed the matrix-induced tube formation of HUVECs inhibited by EA.

A. and B. Cells were treated with 5 µM ellagic acid in the presence of ZnCl₂ ranged from 0–100 µM for 24 hours. C. Cells were treated with or without 100 µM ZnCl₂ for 24 hours and processed for tube formation analysis. D. Cells were treated with 5 µM EA in the presence of 100 µM Zn(C₂H₃O₂)2, Ca Cl2 or MgCl2 for 24 hours and processed for tube formation analysis. The number of completely formed tubes in each group was determined and compared. Values are mean (n = 3) ± SEM. An asterisk demonstrated the significant difference (p<0.05) between none-treated and EA-treated groups.