FIGURE 1.

Illustration and characterization of surfaces. A. Representation of IgE-primed cells contacting different types of antigen presenting surfaces. B. Tracking of fluorescent IgE bound to 5 mol%, 25 mol% DNP-lipid/POPC lipid bilayers and crosslinked DNP-BSA surfaces. Arrows indicate starting point and initial direction of each track. Diffusion coefficients were calculated from tracking of IgE^AF488 bound to each tested surface (Table at right). C. Sequential images of FRAP performed on a 25 mol% bilayer showing pre-bleach, bleach spot, and recovery. Calculated diffusion coefficients for each surface are indicated in the associated table. *Statistically significant with P-value ≤ 0.01; n=5.