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Effects of ABC294640 or sorafenib on autophagy markers in HCC cells. SK-Hep-1, HepG2 or Hep 3b2.1-7 cells were exposed to ABC294640 or sorafenib for 48 hr, except where indicated. Cell lysates were then fractionated by SDS-PAGE, and probed with antibodies to detect LC3-II, beclin-1, actin, p-ERK or ERK as described in the Materials and Methods section.
