Fig. 1.
Expression of recombinant adenovirus-mediated soluble ST2 (Ad-sST2-Fc) in vivo. (a) Ad-sST2-Fc was administered intranasally to BALB/c mice at a dose of 5 × 108 plaque-forming units (pfu)/mice. Time–course of sST2-Fc expression in bronchoalveolar lavage fluid (BALF) was measured by enzyme-linked immunosorbent assay (ELISA). (b) Animals were treated with different virus doses (high: 1 × 109 pfu/mice; medium: 5 × 108 pfu/mice; low: 1 × 108 pfu/mice). Bronchoalveolar lavage was performed 2 days later after viral administration and sST2-Fc expression in BALF was determined by ELISA. Data are presented as the mean ± standard error of the mean (n = 4–6 in each group); n.s.: not significant.