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High levels of soluble ST2 (sST2-Fc) in vivo inhibit nuclear factor-κB activation. Mice werepretreated with adenovirus-mediated sST2-Fc (Ad-sST2-Fc) following lipopolysaccharide (LPS) challenge. Nuclear extracts were prepared at 3 h from the lung tissues and subjected to electrophoretic mobility shift assay. Assay shown is representative of three experiments with similar results.
