Fig. 1.

Analysis of resting burst and Escherichia coli-induced respiratory burst in neutrophils. Representative fluorescence activated cell sorter (FACS) plots and histograms of neutrophil respiratory burst in (a) a healthy control with virtually no resting burst [0·1% reactive oxygen species (ROS)-producing cells]; (b) a non-infected patient with liver cirrhosis with an increased basal fraction of ROS-producing neutrophils (0·8%) and increased basal ROS release (MFI 44·2); (c) a healthy control after in vitro incubation with opsonized E. coli; and (d) a non-infected patient with liver cirrhosis after in vitro incubation with opsonized E. coli showing an increased fraction (89·7%) and ROS release (MFI 106). FSC-H: forward-scatter; SSC-H: side-scatter; FoP: frequency of parents; FL1-H: fluorescence intensity; M1: non-activated cell population; M2: activated cell population.