Table 2.
Respiratory burst in (a) neutrophils and (b) monocytes
| Median (range) | P-value | ||||
|---|---|---|---|---|---|
| Control, no cirrhosis (n = 29) | Group A cirrhosis, no infection (n = 39) | Group B cirrhosis, infection (n = 45) | Control versus group A | Group A versus group B | |
| (a) Neutrophil stimulation | |||||
| Basal | |||||
| ROS-producing cells (%) | 0·5 (0·0–13·2) | 1·0 (0·0–10·9) | 1·3 (0·0–19·5) | 0·004 | 0·425 |
| MFI | 23·5 (14·6–77·0) | 30·3 (16·0–52·2) | 27·0 (12·3–43·1) | 0·003 | 0·117 |
| iMFI | 0·1 (0·0–3·4) | 0·3 (0·0–4·9) | 0·4 (0·0–6·4) | 0·001 | 0·816 |
| fMLP | |||||
| ROS-producing cells (%) | 1·7 (0·3–35·1) | 2·7 (0·6–18·5) | 2·5 (0·0–22·5) | 0·068 | 0·477 |
| MFI | 22·6 (15·8–27·8) | 25·2 (17·8–42·3) | 25·0 (16·7–37·8) | 0·006 | 0·914 |
| iMFI | 0·4 (0·1–7·4) | 0·8 (0·1–7·8) | 0·6 (0·0–6·6) | 0·018 | 0·315 |
| Opsonized E. coli | |||||
| ROS-producing cells (%) | 98·1 (34·6–99·9) | 98·9 (58·1–100·0) | 97·7 (19·9–100·0) | 0·294 | 0·224 |
| MFI | 50·9 (26·8–99·6) | 67·7 (21·8–257·0) | 43·7 (17·2–161·0) | 0·020 | 0·010 |
| iMFI | 50·3 (10·0–98·6) | 65·2 (16·9–257·0) | 43·5 (3·7–144·9) | 0·026 | 0·014 |
| PMA | |||||
| ROS-producing cells (%) | 100 (74·2–100·0) | 100 (23·7–100·0) | 99·9 (56·5–100·0) | 0·881 | 0·187 |
| MFI | 192·0 (22·5–347·0) | 228·0 (18·3–431·0) | 194·0 (16·6–433·0) | 0·385 | 0·062 |
| iMFI | 192·0 (16·7–347·0) | 227·5 (4·3–429·3) | 193·8 (10·7–433·0) | 0·382 | 0·058 |
| (b) Monocyte stimulation | |||||
| Basal | |||||
| ROS-producing cells (%) | 1·1 (0·0–18·5) | 1·3 (0·0–31·5) | 2·3 (0·0–19·0) | 0·423 | 0·326 |
| MFI | 16·3 (12·7–26·9) | 16·7 (13·3–38·2) | 15·4 (14·1–23·0) | 0·963 | 0·072 |
| iMFI | 0·2 (0·0–2·8) | 0·3 (0·0–6·1) | 0·4 (0·0–6·0) | 0·395 | 0·492 |
| fMLP | |||||
| ROS-producing cells (%) | 3·0 (0·0–18·0) | 2·2 (0·0–42·0) | 4·0 (0·0–18·5) | 0·708 | 0·350 |
| MFI | 18·2 (15·4–24·2) | 16·8 (13·9–22·3) | 16·1 (13·0–22·5) | 0·014 | 0·579 |
| iMFI | 0·6 (0·0–3·5) | 0·4 (0·0–6·9) | 0·7 (0·0–3·2) | 0·559 | 0·474 |
| Opsonized E. coli | |||||
| ROS-producing cells (%) | 91·2 (19·0–99·6) | 94·3 (62·5–100·0) | 92·1 (11·8–100·0) | 0·014 | 0·103 |
| MFI | 20·3 (15·8–34·6) | 25·3 (16·0–50·9) | 20·7 (13·8–38·9) | 0·007 | 0·091 |
| iMFI | 18·4 (3·3–32·6) | 22·4 (10·0–50·9) | 20·1 (1·8–38·9) | 0·004 | 0·080 |
| PMA | |||||
| ROS-producing cells (%) | 99·9 (40·9–100·0) | 100·0 (6·4–100·0) | 99·3 (2·6–100·0) | 0·889 | 0·071 |
| MFI | 28·3 (16·3–49·5) | 33·3 (14·8–62·8) | 29·7 (13·9–96·5) | 0·031 | 0·231 |
| iMFI | 28·3 (6·7–49·5) | 33·3 (1·0–62·8) | 28·7 (0·4–96·5) | 0·039 | 0·192 |
MFI: mean fluorescence intensity; iMFI: integrated mean fluorescence intensity; fMLPL N-formyl-methionyl-leucyl-phenylalanine; PMA: phorbol-12-myristate-13-acetate; ROS: reactive oxygen species.