Fig. 4.

Increased expression of granulocyte–macrophage colony-stimulating factor receptor (GM-CSFR) by Gr1⁺CD11b⁺ cells in dextran sulphate sodium (DSS)-treated spleens. Total RNA from splenic Gr1⁺CD11b⁺ cells isolated at day 11 was extracted, DNase-treated and converted to cDNA. Real-time polymerase chain reaction (PCR) was performed according to the Materials and methods. Bars indicate mean values of three experiments. Normal indicates untreated control.