Figure 1.

PDRG expression is selectively upregulated by genotoxic stress. (A) HCT15 and NIH3T3 cells were untreated as control (C) or treated with 2 µM adramycin (Adr), 30 µM etoposide (Etop) or 20 J/m² ultraviolet radiation (UV) for 24 hours. Total RNA preparation and northern blot analyses were done as described in Material and Methods. A human PDRG cDNA probe was used for the blot contains RNA from HCT15 cells, and a mouse PDRG cDNA probe was used for the blot containing RNA from NIH3T3 cells. The integrity of total RNA and comparable loading are shown by ethidium bromide staining of the gel prior to transfer. (B) DU145 and HS578T cells were untreated as control (C) or treated with adramycin (Adr), etoposide (Etop), camtothecin (Cam), Taxol, thapsigargin (TG) or sulindac sulfide (SD) and then harvested after 24 hours or at indicated time points. Total RNA was extracted and northern blot analyses of PDRG mRNA levels were done using human PDRG cDNA probe as described in Material and Methods. Ethidium bromide staining of the gel shows integrity of RNA and comparable loading.