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. 2011 Mar 15;11(6):567–573. doi: 10.4161/cbt.11.6.14412

Figure 6.

Figure 6

PDRG interacts with PDCD7 in mammalian cells. HE K 293T cells were transiently transfected with expression vector pCEP-PDRG1 and pCEP-PDCD7 or pCEP-PDRG1 and pCEP 4 vector without PDCD7 insert. After 24 hours, cells were harvested and lysed. About 500 total lysates were precipitated with anti-myc antibody (9E10) and protein G-bead. The precipitated proteins were resolved by SDS-PAGE along with 150 µg total cell lysates. HA-tagged PDRG was detected by western blotting with rat monoclonal anti-HA antibody and myc-tagged PDCD7 was detected using mouse monoclonal anti-myc antibody (9E10).