Skip to main content
. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: Mol Cell. 2011 Apr 14;42(2):147–159. doi: 10.1016/j.molcel.2011.03.005

Figure 5. DAPK1 suppresses Pin1-induced centrosome amplification.

Figure 5

(A and B) S71D, but not S71A abolishes the ability of Pin1 to induce centrosome amplification. NIH3T3 cells stably expressing Pin1, its Ser71 mutants or control were arrested at the G1/S boundary by aphidicolin. Cells were stained with anti-γ-tubulin antibody (red) and DAPI (blue). Bar, 10 μm (A). Cells containing >2 centrosomes were scored in 300 transfected cells (B). Results shown are mean ± SEM, n = 3. **, p <0.001.

(C and D) DAPK1 inhibits Pin1-induced centrosome amplification. Stable cell lines were arrested at the G1/S boundary by aphidicolin in the presence or absence of doxycycline. Cells were stained with anti-γ-tubulin antibody (red) and DAPI (blue). Bar, 10 μm (C). Cells containing >2 centrosomes were scored in 300 transfected cells (D). Results shown are mean ± SEM, n = 3.

HHS Vulnerability Disclosure