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. 2011 Apr 20;31(16):6174–6187. doi: 10.1523/JNEUROSCI.5464-10.2011

Figure 7.

Figure 7.

GN11 cells express Robo receptors and respond to class 3 semaphorins and Slit1. A, Reverse transcription-PCR analysis showed expression of Robo1–3 in GN11 cells. B, Immunohistochemistry confirmed the expression of Robo receptors in GN11 cells. C, D, Quantification of GN11 cell migration in a Boyden's chamber, containing Sema3A, Sema3F, Slit1, or control (CM-myc) conditioned media (C). D, Robo1-DN-transfected GN11 cells are less responsive to Sema3A than control (GFP)-transfected cells. Scale bar, 100 μm. ***p < 0.001. Error bars indicate SEM.