Table 1. MICs of Gyramides (R)-1, 2, and 3 and (S)-1^a.

bacterial strain	(R)-1	2	3	(S)-1
E. coli BW25113 ΔtolC	10	5	2.5	140
E. coli BW25113 + 60 μM MC-207,110	40	40	20	ND
P. aeruginosa K + 60 μM MC-207,110	80	40	20	ND
S.enterica ATCC 19585 + 60 μM MC-207,110	80	20	10	ND
S. aureus FRI 100	>160	80	20	ND
S.pneumoniae ATCC 10813	160	20	10	ND
E.faecalis 1131	>160	>160	>160	ND

^aThe MICs of the gyramides were determined for the drug pump knockout strain E. coli BW25113 ΔtolC. We used 60 μM drug pump inhibitor MC-207,110 with the wild type strains E. coli BW25113, P. aeruginosa K, and S. enterica ATCC 19585 to potentiate the activity of the compounds. The susceptibility of the wild type strains of S. aureus FRI100, S. pneumoniae ATCC 10813, and E. faecalis 1131 without a pump inhibitor are shown. The label “ND” indicates data that we did not collect, as the activity of (S)-1 was low against E.coli BW25113 ΔtolC. The values in the table are reported in μM concentrations.