Fig. 4.

Histopathological assessment of liver damage during P. berghei ANKA (PbA) infection. C57BL/6 mice (n = 5 or 6) were infected with P. berghei ANKA-luc or left naïve. (A) At 5 or 6 days after infection, liver-resident CD8⁺ T cells were enumerated by flow cytometry. *, P < 0.05; **, P < 0.01 (Mann-Whitney test, relative to naïve group). (B) At 5 or 6 days after infection, paraffin-embedded tissues were sectioned and stained with hematoxylin and eosin (upper panels) or with picrosirius red (lower panels). Collagen is depicted by red staining and malaria pigment by punctuate black staining in the lower panels. Bars, 10 μm. These data are representative of two independent experiments performed.