Fig. 4.

Downstream signals affected by EGFR activation by P. fluorescens. (A and C) A549 cells were pretreated with vehicle (dimethyl sulfoxide [DMSO]) or 10 μM AG1478 for 2 h and were then infected with P. fluorescens for the indicated times. Protein lysate from A549 cells infected with P. fluorescens was subjected to Western blot analysis. (B) A549 cells grown to 90% confluence were infected with P. fluorescens at a cell-to-bacterium ratio of 1:50 for each time period. Cell lysate of each sample was subjected to Western blot analysis.