Fig. 3.

Phenotypic characterization of splenic transgene-specific CD8⁺ T cells elicited by heterologous plasmid DNA priming-recombinant HuAd5 boosting vaccination regimen 14 or 98 days after the last immunizing dose. C57BL/6 mice were immunized according to the schedule presented in Fig. 1A. Fourteen or 98 days after the recombinant HuAd5 dose, splenic cells were stained with anti-CD8, H-2K^b/VNHRFTLV, and the indicated marker-specific antibody prior to analysis by FACS. The histograms show the expression of the markers on CD8⁺ H2K^b/VNHRFTLV⁺ T cells (blue lines) or control naive CD8⁺ spleen cells (red lines). Analyses were performed using pools of cells from three mice and are representative of two or more experiments performed independently. Numbers indicate the mean fluorescence intensities. In the case of KLRG-1, percentages of positive cells are indicated.