Fig. 5.

Effects of IFN-γ and IL-4 deletion on numbers of Ym1/2-expressing CD11b⁺ cells in C. neoformans-infected lungs. (A) CD11b-expressing cells were isolated from C. neoformans-infected WT, IL-4^−/−, and IFN-γ^−/− mice (3 wpi) by MACS separation and counted under a microscope. The data are means from 3 independent experiments, with ≥3 mice/group/experiment. (B) CD11b-selected cells were stained with Ym1/2 Ab to enumerate Ym1/2-positive and -negative cells. Approximately 100 cells were counted in 10 different fields on a slide. **, P < 0.005 for the IL-4^−/− group compared to the WT group; ††, P < 0.005 for the IL-4^−/− group compared to the IFN-γ^−/− group. Note that the total number of CD11b-selected cells was not affected by IL-4 or IFN-γ deletion but that there was a strong effect on the numbers of cell staining positive for Ym1/2.