Fig. 2.

Biochemical activities of recombinant LegK proteins. (A) SDS-PAGE analysis of purified GST-LegK1, GST-LegK2, GST-LegK3, 6His-LegK4, and 6His-LegK5 after staining with Coomassie blue. Molecular mass standards are indicated on the left. (B) Effects of cations on autokinase activities of GST-LegK2, GST-LegK3, 6His-LegK4, and 6His-LegK5. Purified LegK proteins were subjected to in vitro autophosphorylation assays in the presence of [γ-³²P]ATP and Mg²⁺ or Mn²⁺. Phosphoproteins were separated by SDS-PAGE and then revealed by autoradiography. (C) Protein kinase activities of LegK proteins. The eukaryotic substrate myelin basic protein (MBP) was incubated with each LegK recombinant protein in the presence of [γ-³²P]ATP. Phosphoproteins were visualized by autoradiography after SDS-PAGE separation.