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Growth-dependent expression of agr. Expression of agr was determined using qRT-PCR of RNAIII during the growth of S. aureus strains and their isogenic agr mutants. Lines represent OD600, plotted on the left y axis. Bars represent the relative expression of RNAIII compared to that of the housekeeping gene gyrB (control), plotted on the right y axis. Expression of RNAIII in agr-negative strains was not detectable in any strain at any time point.
