Effect of agr on the expression of selected surface protein and regulatory genes in various strain backgrounds. The graphs show expression of selected genes as determined by qRT-PCR at maximal expression of agr in the LAC, LAC agr, RN6390, and RN6911 strains for 8 h and the 252 and 252 agr strains for 10 h. Data shown on the y axis represent expression relative to that of the housekeeping gene gyrB (control). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (t tests comparing agr-negative to wild-type strain samples). Expression levels of alpha-toxin were confirmed on the protein level by Western blots with specific alpha-toxin antiserum and are shown above the qRT-PCR data.