Fig. 4.

PCR analysis confirmed that the wild-type, plzA mutant, and the complemented plzA⁺ cells retained endogenous plasmids required for infection of mice and ticks. Twenty-one sets of PCR primers were used to detect 12 linear (lp) and 9 circular (cp) B. burgdorferi plasmids (12, 100). Arrows indicate the cp9 and lp56 plasmids were lost from all strains.