Fig. 5.

Swarm plate motility assays confirmed that the plzA mutant was defective in motility. A representative swarm assay with plzA mutant cells incubated for 7 days is shown. Since the swarm diameters were not clearly visible, a black bar was placed on each swarm, which represents the size of their swarm diameters (left panel). Swarm diameters from 4 assays were measured in millimeters ± standard deviations (right panel). A flagellinless, nonmotile flaB mutant was used as a control (51). *, significant difference (P = 0.001) between wild-type (WT) and plzA mutant cells.