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Time course experiment showing expression of PD-1 in spleen cells from mice infected with T. cruzi. Spleen cells were obtained from mice at different time points after infection with T. cruzi, and the expression of PD-1 was determined in each cell subset by cytometry. (A to F) Gated on lymphocytes (A to D) and on monocytes (E, F). Data are representative values obtained from three independent experiments. Asterisks denote P values of <0.005 by ANOVA compared to uninfected values (0).
