Fig. 3.

Expression of PD-1 in T cells infiltrating the myocardium during T. cruzi infection. Heart tissues from infected mice (20 dpi) were processed to isolate inflammatory cells, and the levels of expression of PD-1 and the ligands PD-L1 and PD-L2 in the gate of T CD4 (B to E) or T CD8 (G to J) cells were assessed by cytometry. (A and F) Representative dots of the ungated cells; (B and G) representative dots of each T cell subset studied. The bar in each histogram represents the expression level of each molecule according to the IgG control (which is shown by a dashed line). Data are representative values obtained from three independent experiments. SSC-H, side scatter; FSC-H, forward scatter.