Table 1.

Comparison of the extents of altered expression of selected genes and operons observed for tobramycin microarrays and resistant mutant screens^e

Gene or operon	Extent of altered regulationa					TOB resistance geneb
	Aerobic WT (TOB at 2 μg/ml)	Anaerobic WT (TOB at 20 μg/ml)	Aerobic WT (TOB at 0.25 μg/ml)	Anaerobic WT (TOB at 2 μg/ml)	Aerobic PA0779 (asrA::lux) (TOB at TOB 2 μg/ml)
Heat shock
htpG-PA1597	+++	+++		+	+
ibpA	++++	++++	+	+	++
groES groEL	++	++			+
dnaJ dapB	++	+++		+	+
grpE dnaK	+++	+++		+	+
hslVU-PA5055	++	+++			+
Energy metabolism
PA1547-PA1551	−	−				Yes
ccoN1O1Q1P1	−	−
ccoN2O2Q2P2	−−	+				Yes
sdhCDAB		−
sucAB lpdG		−
sucCD		−				Yes
PA1600-PA1602	−−		−−−		−
nuoABDEFGHIJKLMN	−	−−−				Yes
nqrABCDEF	−	−				Yes
Denitrification/nitrogen metabolism
nirSMCFDLH-PA0512-nirJEN	−−	−−	−		−
nirQ-PA0521-PA0522		+
norCB		+	−		−
norD		−				Yes
nosRZDFYL	−	−−	−−		−	Yes
narK1K2GHJI-PA3871	−	−−−	−−−		−−
narXL	−			+
Other
asrA	++	++		+
pqsABCDEphnA			+
mexXY			+++
hcnABC	−	−	+
Pilus operonsc	−	−−
Flagellin operonsd	−	−

^a−, downregulated 2- to 4-fold; −−, downregulated 5- to 10-fold; −−−, downregulated ≥10-fold; +, upregulated 2- to 9-fold; ++, upregulated 10- to 20-fold; +++, upregulated 20- to 50-fold; ++++, upregulated ≥50-fold. TOB, tobramycin; WT, wild type.

^bDetermined to be resistant in a screen of inactivation mutants reported previously by Schurek et al. (39).

^cpilJK chpABCD pilB pilCD coaE-PA4530 fimU pilVWXY1Y2E PA4958-fimX.

^dflgFGHIJK PA1442-fliMNOPQR flhB.

^eThe full list of dysregulated genes can be found in Table S1 in the supplemental material.