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. 2011 May;55(5):2466–2468. doi: 10.1128/AAC.01548-10

Fig. 1.

Fig. 1.

Emergence of linezolid-resistant subclones by Etest and their population analysis. (A) Etest. Cells were grown in drug-free MH broth overnight, and a 100-μl aliquot was streaked on MH-blood agar with a linezolid-impregnated Etest strip. Plates were incubated at 35°C for 48 h. HG503pre (a), HG503post (b), and randomly selected HG503post cells from the drug-free agar medium (c and d) were subjected to Etest. Only two subclones are shown. (B) Population analysis. Cells were grown overnight in drug-free MH broth, and 100-μl aliquots were streaked on BHI agar impregnated with various concentrations of linezolid. The numbers of colonies on the plates were counted after incubation at 35°C for 48 h. (a) HG503pre. (b) HG503post.