Table 3.
Details of percent FOSC cell subpopulations measured in a flow cytometer using CMFDA and Sytox Red after exposure to ML and OP
| Cell type, strain, and fluorescence characteristic | Mean % cell subpopulation ± SD |
|||||||
|---|---|---|---|---|---|---|---|---|
| ML |
OP |
|||||||
| 15 min | 30 min | 60 min | 240 min | 15 min | 30 min | 60 min | 360 min | |
| Planktonic cells | ||||||||
| MYC-158-06 | ||||||||
| CMFDA+/Sytox Red− | 44 ± 5.3 | 3 ± 4.5 | 1 ± 0.6 | 0 | 62 ± 7.9 | 30 ± 8.7 | 10 ± 4.6 | 8 ± 1.1 |
| CMFDA+/Sytox Red+ | 55 ± 4.9 | 91 ± 4.1 | 92 ± 3.1 | 1 ± 0.2 | 32 ± 4.5 | 50 ± 9.8 | 30 ± 7.6 | 13 ± 4.5 |
| CMFDA−/Sytox Red+ | 1 ± 0.4 | 3 ± 0.6 | 3 ± 0.7 | 96 ± 5.6 | 6 ± 2.3 | 20 ± 4.8 | 60 ± 8.9 | 79 ± 10.1 |
| MYC-159-06 | ||||||||
| CMFDA+/Sytox Red− | 48 ± 7.1 | 15 ± 5.6 | 6 ± 0.4 | 0 | 65 ± 4.6 | 33 ± 7.5 | 15 ± 2.3 | 7 ± 1.4 |
| CMFDA+/Sytox Red+ | 50 ± 6.9 | 82 ± 4.2 | 22 ± 5.6 | 14 ± 1.1 | 29 ± 4.7 | 49 ± 4.8 | 16 ± 5.6 | 11 ± 4.1 |
| CMFDA−/Sytox Red+ | 2 ± 0.9 | 3 ± 0.9 | 72 ± 4.9 | 86 ± 7.9 | 6 ± 2.5 | 18 ± 2.3 | 69 ± 4.2 | 82 ± 4.4 |
| MYC-398-06 | ||||||||
| CMFDA+/Sytox Red− | 38 ± 7.1 | 18 ± 5.4 | 9 ± 2.3 | 0 | 45 ± 4.5 | 24 ± 3.1 | 17 ± 2.1 | 9 ± 2.1 |
| CMFDA+/Sytox Red+ | 59 ± 6.7 | 78 ± 5.5 | 42 ± 4.5 | 38 ± 4.4 | 53 ± 3.0 | 59 ± 3.5 | 68 ± 5.1 | 9 ± 2.5 |
| CMFDA−/Sytox Red+ | 3 ± 1.0 | 4 ± 0.8 | 49 ± 4.4 | 62 ± 5.6 | 2 ± 0.7 | 17 ± 1.8 | 15 ± 2.5 | 79 ± 6.4 |
| Biofilm cells | ||||||||
| MYC-158-06 | ||||||||
| CMFDA+/Sytox Red− | 50 ± 6.3 | 47 ± 3.5 | 22 ± 2.5 | 9 ± 1.5 | 62 ± 4.5 | 45 ± 3.5 | 26 ± 2.0 | 14 ± 2.0 |
| CMFDA+/Sytox Red+ | 15 ± 2.1 | 24 ± 1.4 | 48 ± 4.7 | 70 ± 2.0 | 30 ± 3.0 | 22 ± 3.0 | 26 ± 4.5 | 17 ± 3.0 |
| CMFDA−/Sytox Red+ | 6 ± 1.3 | 9 ± 1.5 | 18 ± 2.5 | 19 ± 1.5 | 8 ± 0.9 | 33 ± 5.9 | 58 ± 7.3 | 70 ± 8.4 |
| MYC-159-06 | ||||||||
| CMFDA+/Sytox Red− | 78 ± 5.8 | 52 ± 6.0 | 36 ± 4.7 | 22 ± 2.4 | 68 ± 9.6 | 39 ± 4.6 | 23 ± 2.1 | 11 ± 4.6 |
| CMFDA+/Sytox Red+ | 22 ± 4.5 | 16 ± 3.5 | 36 ± 4.4 | 10 ± 1.5 | 27 ± 6.2 | 33 ± 2.3 | 36 ± 4.5 | 15 ± 7.2 |
| CMFDA−/Sytox Red+ | 0 | 32 ± 2.1 | 28 ± 1.4 | 78 ± 8.7 | 5 ± 1.0 | 28 ± 2.5 | 41 ± 8.7 | 72 ± 7.6 |
| MYC-398-06 | ||||||||
| CMFDA+/Sytox Red− | 66 ± 6.3 | 48 ± 3.5 | 25 ± 5.9 | 11 ± 1.7 | 71 ± 4.9 | 43 ± 8.9 | 22 ± 4.8 | 9 ± 4.5 |
| CMFDA+/Sytox Red+ | 30 ± 4.2 | 36 ± 2.1 | 36 ± 1.7 | 20 ± 5.8 | 18 ± 3.9 | 36 ± 9.5 | 45 ± 8.7 | 12 ± 6.4 |
| CMFDA−/Sytox Red+ | 4 ± 2.1 | 16 ± 4.6 | 39 ± 1.1 | 69 ± 7.9 | 11 ± 1.8 | 21 ± 7.8 | 33 ± 7.7 | 85 ± 7.9 |