Fig. 3.

Metabolism of GS-9191 in HeLa cells following CatA knockdown using siRNA. HeLa cells were mock transfected (○) or transfected with CatA-specific (▾) or control (●) siRNAs. The cells were incubated with ¹⁴C-labeled GS-9191 (10 μM; 0.521 μCi/ml) for 0 to 4 h on day 3. At selected time points, medium was removed, and the intracellular concentration of the metabolites was determined as described in Materials and Methods. The data are expressed as means ± standard deviations of two independent experiments.